The activities of IL-la and TNFα to activate human peripheral blood mononuclear cells (PBMC) to inhibit albicans were studied.
PBMC were isolated from normal human peripheral blood. Adherent PBMC were allowed to adhere to flat bottom wells of microtiter plates. Yeast cells of Candida albicans (effect/target cell ratio:1 or 100) were added in the presence of IL-la, TNFα
20%
normal human serum or no cytokines and plates were incubated at 37℃, 5% CO2- After 3hrs or 24hrs incubation, killing was determined by comparing the quantitative plate counts of viable yeast cells in experimental wells counts in control wells
with
containing yeast cells in the abcence of PBMC, adherent and nonadherent PBMC.
Nonadherent PBMC killed C. albicans more effectively than adherent cells. PBMC stimulated with IL-la, a concentration of 100μ/ml, showed no high effective candidacidal activity compared with PBMC without cytokines. But PBMC stimulated with IL-la
or
TNFα, concentration of 1000μ/ml, showed high candidacidal activity. But PBMC with TNFα showed more effective candidacidal activity than lL-la.
PBMC was remarkably more effective in killing to opsonizing C. albicans with of 20% human serum than stimulated with the IL-lα. When adherent PBMC stimulated for Ohr(coincubation), 24hrs, 3, or 7 days with TNFαprior to target cells challenge,
highest
candidacidal activity appeared at 24hrs stimulation.
|